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Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas ArchiMer
Da Costa Gonzalez, Fiz; Robert, Rene; Quere, Claudie; Wikfors, Gary H.; Soudant, Philippe.
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5...
Tipo: Text Palavras-chave: Fatty acid; Larvae; Lipids; Metabolism; Oyster; Synthesis.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00268/37962/36086.pdf
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Etude comparative des caractéristiques écophysiologiques et des performances de croissance de l'huître creuse Crassostrea gigas diploïde et triploïde en milieu contrôlé ArchiMer
Haure, Joel; Fortin, Adeline; Dupuy, Beatrice; Nourry, Max; Palvadeau, Hubert; Papin, Mathias; Penisson, Christian; Martin, Jean-louis.
Introduction: Marine farming in France is currently very widely dominated by oyster farming. French oyster production totals 148,467 tons, for a turnover of close to 280 million euros, making France the leading European producer. The two main species concerned are the cup oyster, Crassostrea gigas (140,000 tons) and the flat oyster, Ostrea edulis (2,000 tons), the production of which has collapsed since 1970 following successive epizootics of Marteilia refringens and Bonamia ostreae. French oyster farming is thus in a situation of quasi single-crop farming, with more than 90% of tonnage pertaining to the cup oyster (FAO data from 1993). This situation of quasi single-crop farming leads to the pessimistic observation that one hypothetical crisis in the...
Tipo: Text Palavras-chave: Growth; Ecophysiology; Oyster; Crassostrea Gigas; Eeproduction; Milieu Controle; Croissance; Triploide; Diploide; Ostreidae; Huitre; Crassostrea Gigas; Ecophysiologie; Reproduction.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/rapport-1509.pdf
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Etude de la structure des communautés bactériennes du sédiment et de l'écologie de Vibrio aestuarianus pathogène de l'huître creuse Crassostrea gigas dans deux sites ostréicoles ArchiMer
Azandegbe, Afi.
Since several years, the French oyster farming copes with high mortality affecting juveniles and some adults of the Pacific oyster Crassostrea gigas. Studies conducted in the framework of a multidisciplinary project (MOREST) showed that mortality was related to several factors like physiological and genetic status of oyster, pathogens including bacteria of the genus Vibrio, and revealed that the sediment, on which the oyster cages are installed, might play a particular role. This has been suggested because of the concomitance of the mortalities and increased toxic compounds like ammonia and sulphides in the sediment. Moreover, the presence of Vibrio bacteria in the sediment indicates a dual role of sediment in the mortality onset. The physico-chemical...
Tipo: Text Palavras-chave: Huître; Crassostrea gigas; Bactéries; Vibrio aestuarianus; Écologie; Sédiment; Ammonium; Sulfures; Oyster; Crassostrea gigas; Bacteria; Vibrio aestuarianus; Ecology; Sediment; Ammonia; Sulphide.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00032/14277/11561.pdf
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Etude de la virulence des Vibrio pathogènes en relation avec la variabilité de la réponse chez l'uître creuse ArchiMer
De Decker, Sophie.
The bacteria of the Vibrio genus are one of the most abundant groups in the aquatic ecosystems. Two species of vibrios, Vibrio splendidus and V. Aestuarianus, have been associated, in some cases, to mortality in flat oyster Crassostrea gigas farms, most of the time during the summer period. This doctoral thesis studies the Vibrio-host interaction and their modulations according to the host's pathogenic virulence and its genetic, physiologic and immunitary parameters. These factors will be characterised by in vitro and in vivo approaches (experimental infection). The characterization of contrasted genetic families according to their sensitivity to these vibrios will enable the implementation of new improvement strategies in oyster farms (prophylactic...
Tipo: Text Palavras-chave: Immunologie; Physiologie; Paramètres génétiques; Virulence; Oyster; Crassostrea gigas; Pathogène; Vibrio.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3431.pdf
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Etude des mécanismes antiviraux développés par les huîtres lors d'infections à virus de type herpès ArchiMer
Olicard, Cecile.
Tipo: Text Palavras-chave: Cytometry; Herpes like virus; Oyster; Antivirus mechanisms.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/rapport-1769.pdf
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Etude d'un élevage intégré bars-huîtres ArchiMer
Buchet, Vincent; Bluteau, A.
Whatever the hydraulic gestion, an épuration of the fishes sewages is possible with the integrated system studied. The main action is situed in the intermediary pond with a significant decrease of nutrients, especially ammonia, and particulate mineral matters. The oyster's pond have no significant action on nutrients, but their action is real on chlorophyll which become limiting factor in 20% renewal system. One of the effluent, system 1 (renewal 10%) had high suspended matter and low nutrient concentrations. The other (renewal 20%) was poor in supcnded matters (chlorophyll). The system 2 give best oyster's growth, close to traditional nursery performances, with an highest mortality (32%). In the first system, suspended matter concentrations are too high...
Tipo: Text Palavras-chave: Valorization; Oyster; Pre ongrowing; Seabass; Integrated rearing; Valorisation; Huître; Prégrossissement; Bar; Elevage intégré.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/rapport-1917.pdf
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Etude et modélisation de la dynamique des populations de bivalves en élevage (Crassostrea gigas et Mytilus galloprovincialis) dans le bassin de Thau (Méditerranée, France) et des ascidies solitaires associées ArchiMer
Gangnery, Aline.
The total standing stock of bivalves cultured in Thau Lagoon (France) that is mainly constituted of the Pacific oyster, Crassostrea gigas, and the Mediterranean mussel, Mytilus galloprovincialis, vary between 14,000 and 20,000 tons within a year. These variations were attributed to the rearing cycle of molluscs in the lagoon but also to their growth performances. Oysters cultured in the Thau Lagoon presented the best growth rate ever reported whereas trophic conditions are low : mean concentrations in organic particulate matter and total chlorophyll a averaged 1 mg.l-1 and 1.2 µg.l-1, respectively. The development of a growth model shows that temporal pattern (i.e. seasonality) of bivalve growth is explained by water temperature and spatial pattern is...
Tipo: Text Palavras-chave: Lagoon ecosystem; Resource management; Rearing strategies; Production; Standing stock; Hydrobiology; Growth; Shellfish culture; Ascidiella aspersa; Mussel; Oyster; Ecosystème lagunaire; Gestion des ressources; Stratégies d'élevage; Production; Stock; Hydrobiologie; Croissance; Conchyliculture; Ascidiella aspersa; Moule; Huître.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/these-102.pdf
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Etudes sur la pathologie des huîtres ArchiMer
Balouet, G..
French summary: In 1974, at the request of a group of oyster farmers in the Brittany region, the Pathology Laboratory of the U.E.R. Medicine of Brest undertook a series of theoretical and applied research projects concerning the problem of flat oyster digestive gland disease, due to Marteilia refringens, the primary economic problem of that time. In 1978, in the research report of Contract C.N.E.X.O. 77/1690, the main results obtained since the beginning of the study of the parasitosis were made public. The present report has as its objective to show: - The results obtained in 1978-1979 while monitoring the parasitosis, and most especially the epidemiological evolution of the disease; - Information concerning the presence of other infectious diseases...
Tipo: Text Palavras-chave: Parasite; Ostrea edulis; Oyster; Pathology.
Ano: 1978 URL: http://archimer.ifremer.fr/doc/1978/rapport-1927.pdf
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Evidence of differential chromosome loss in aneuploid karyotypes of the Pacific oyster, Crassostrea gigas ArchiMer
Leitao, Alexandra; Boudry, Pierre; Thiriot Quievreux, Catherine.
The G-banding technique was performed on aneuploid karyotypes from gill tissue of the Pacific oyster, Crassostrea gigas, to assess whether chromosome losses could be explained by differential chromosomal susceptibility and to clarify the negative correlation between aneuploidy and growth rate previously reported in different populations of this oyster. The study of 95 G-banded aneuploid karyotypes showed that only 4 of the 10 chromosome pairs (viz. 1, 5, 9, and 10) of C. gigas were affected by the loss of one homologous chromosome. Pairs 1, 9, and 10, which were lost in 56, 33, and 44% of cases, respectively, may be considered to be differentially affected. Hypotheses on this differential chromosomal susceptibility are discussed.
Tipo: Text Palavras-chave: Oyster; Karyotype; G banding; Growth; Aneuploidy; Huître; Caryotype; Bandes G; Croissance; Aneuploïdie.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-742.pdf
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Evidence of herpesvirus (OsHV-1) resistance in juvenile Crassostrea gigas selected for high resistance to the summer mortality phenomenon ArchiMer
Degremont, Lionel.
During the MOREST project (2001–2006), oyster summer mortality was extensively investigated in Crassostrea gigas in France. However, significant increases in magnitude and geographic distribution of summer mortality episodes have been reported since 2008. This paper investigates the response to selection for survival, which was successfully performed between 2001 and 2003, in this new context. It also examines the relationship between the mortality and the three pathogens Vibrio aestuarianus, V. splendidus and the Ostreid herpesvirus 1 (OsHV-1). Three batches of juvenile (6-month-old) Crassostrea gigas were produced in February 2009 and tested in the field in the Marennes-Oléron Bay during summer (August and September) 2009: one control batch, one batch...
Tipo: Text Palavras-chave: Summer mortality; Crassostrea gigas; Disease resistance; Ostreid herpesvirus; Vibrio sp; Oyster.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00033/14472/11770.pdf
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Experimental and natural cathodoluminescence in the shell of Crassostrea gigas from Thau lagoon (France): ecological and environmental implications ArchiMer
Langlet, D; Alunno-bruscia, Marianne; Rafelis, M; Renard, M; Roux, M; Schein, E; Buestel, Dominique.
We present a cathodoluminescence (CL) study of growth layer deposition in the shell of the oyster Crassostrea gigas. CL is based on the physical properties of lattice-bound manganese (Mn2+), which is the main activator in calcium carbonate. Our study involved chemical marking by immersing individuals in seawater to which manganese chloride had been added, and subsequent reading of the shell with CL microscopy coupled with numeric treatment of microphotographs; CL emission was analyzed using a scanning electron microscope coupled to a spectrometer. Since the marking did not harm the oysters, repeated markings were possible, allowing validation of the inferences made from analysis of the shell rings. Oysters reared in a French Mediterranean lagoon (Thau...
Tipo: Text Palavras-chave: Mediterranean lagoon; Manganese marking; Carbonate biomineraliation; Shell growth; Cathodoluminescence; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1979.pdf
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Experimental evidence for a genetic basis to differences in aneuploidy in the Pacific oyster (Crassostrea gigas) ArchiMer
Leitao, Alexandra; Boudry, Pierre; Mccombie, Helen; Gerard, Andre; Thiriot-quievreux, Catherine.
Aneuploidy has been previously reported in wild and cultivated Pacific oysters, Crassostrea gigas, and has been shown to be negatively correlated with growth. This is especially important since high variability of growth rate is one of the major problems in the aquacultural production of this species. The existence of a genetic basis for the observed differences in aneuploidy was first investigated through the comparative study of six full-sib families, with mean individual weights ranging from 0.59 to 1.49 g. The slowest growing family was found to have the highest level of aneuploidy. Significant differences in aneuploidy were also found among families when individuals with the same growth rate were sampled. This supports the hypothesis of the existence...
Tipo: Text Palavras-chave: Oyster; Growth; Genetics; Full sib families; Crassostrea gigas; Aneuploidy; Huître; Croissance; Génétique; Famille de plein frères; Crassostrea gigas; Aneuploïdie.
Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-2700.pdf
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Farmed bivalve loss due to seabream predation in the French Mediterranean Prevost Lagoon ArchiMer
Richard, Marion; Forget, Fabien; Mignucci, Alexandre; Mortreux, Serge; Le Gall, Patrik; Callier, Myriam; Weise, Am; Mckindsey, Cw; Bourjea, Jerome.
Bivalve predation by seabream has been observed worldwide and is a major concern for bivalve farmers. Farmed bivalve-seabream interactions must be better understood to ensure the sustainability of bivalve aquaculture. The objectives of this study were to characterize gilthead seabream Sparus aurata presence in a bivalve farm in Prevost Lagoon (Mediterranean Sea) using acoustic telemetry and to evaluate monthly losses of mussels Mytilus galloprovincialis and oysters Crassostrea gigas due to seabream predation over an 18 mo period inside the farm and at an unprotected experimental platform. Large (281 to 499 mm TL) seabream were more commonly detected in the bivalve farm than were small (200 to 280 mm TL) seabream. In contrast to small seabream, 90% of large...
Tipo: Text Palavras-chave: Shellfish culture; Sparus aurata; Size; Acoustic telemetry; Crassostrea gigas; Mytilus galloprovincialis; Oyster; Mussel.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00663/77515/79299.pdf
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Feeding of Crassostrea gigas at high seston load : modeling requirements ArchiMer
Barille, Laurent; Prou, Jean.
Ecophysiological experiments conducted in situ in the Bay of Marennes-Oléron during a spring and neap tide cycle in May 1991 , were designed to get a better representation of 2 important components of the energy budget of the Japanese oyster Crassostrea gigas : clearance rate and selection efficiency. The relationships previously established by Raillard et al. ( 1993) are re-evaluated by integrating the effects of high seston loads, typical of tidal effect within estuaries, on the physiological processes. For the clearance rate, the re-evaluated parameterization includes: - a negative effect of turbidity. - a clogging threshold set to 192 mg/l. Selection efficiency parameterizations are modified by incorporating : - a constant inorganic ingestion for...
Tipo: Text Palavras-chave: Matematical model; Oyster; Crassostrea gigas; Clearance rate; Seclection efficency; Marennes Oleron; Charente Maritime.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00102/21299/18913.pdf
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Feeding rate of the oyster Crassostrea gigas in a natural planktonic community of the Mediterranean Thau Lagoon ArchiMer
Dupuy, Christine; Vaquer, André; Lam Hoai, Thong; Rougier, Claude; Mazouni, Nabila; Lautier, Jacques; Collos, Yves; Le Gall, Solange.
The Mediterranean Thau Lagoon is an important oyster farming area in Europe. Oyster growth rates are among the highest in France, although chlorophyll a concentration is low. Previous studies have demonstrated that picophytoplankton, nano-microphytoplankton, dinoflagellates and loricate ciliates such as tintinnids are abundant. However, heterotrophic flagellates and aloricate ciliates have not been investigated. The aim of this study was to assess picophytoplankton, protist and zooplankton abundances in the Lagoon and to investigate the particular structure of the microbial food web, which may explain such paradoxical oyster growth. In oligotrophic waters in the Thau Lagoon, the picoeukaryote Ostreococcus tauri is the dominant autotrophic picoplankter,...
Tipo: Text Palavras-chave: Trophic link; Picophytoplankton; Heterotrophic protist; Microbial food web; Thau Lagoon; Food source; Oyster; Bivalve.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-812.pdf
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First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway ArchiMer
Montagnani, Caroline; Avarre, Jean-christophe; De Lorgeril, Julien; Quiquand, M; Boulo, Viviane; Escoubas, Jean-michel.
In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and...
Tipo: Text Palavras-chave: Damage associated molecular pattern; Tissue inhibitor of metalloproteinase; Metalloproteinase; Mollusk immunity; Crassostrea gigas; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2094.pdf
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First results of an epidemiological study on oyster (Crassostrea gigas) mortality events in France during summer 2008 ArchiMer
Miossec, Laurence; Allain, Gwenhael; Arzul, Isabelle; Francois, Cyrille; Garcia, Celine; Cameron, Angus.
Abnormal mortalities occurred in most oyster producing areas in France during the summer of 2008. The mortalities were sudden and severe (up to 100%) and mainly affected 6 to 18 month old juveniles. Only Crassostrea gigas species was affected. The cause of the mortality was unclear but OsHV-1 virus and bacteria belonging to Vibrionaceae family were frequently detected in affected populations. An epidemiological study was commenced in autumn 2008 in order to identify factors associated with, and the cause (or causes) of these mortalities. This paper presents the results of the first (descriptive) part of this investigation. The objectives were to identify the affected population and to describe the pattern of mortalities in time and space. Mortality...
Tipo: Text Palavras-chave: France; Crassostrea gigas; Oyster; Mortality; Epidemological study.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/acte-6772.pdf
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Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae ArchiMer
Gervais, Ophelie; Chollet, Bruno; Renault, Tristan; Arzul, Isabelle.
The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses....
Tipo: Text Palavras-chave: Apoptosis; Host-parasite interactions; Parasite; Oyster; Bonamia ostreae; Ostrea edulis.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00346/45692/45312.pdf
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Flow cytometry to measure impact of temperature and salinity on the survival of Bonamia ostreae, parasite infecting flat oyster Ostrea edulis, in seawater ArchiMer
Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979ı in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely...
Tipo: Text Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf
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Fluctuating asymmetry and mortality in cultured oysters (Crassostrea gigas) in Marennes-Oleron basin ArchiMer
Frechette, Marcel; Goulletquer, Philippe; Daigle, Gaétan.
Japanese cupped oysters suffer summer mortality in many culture sites along the French Atlantic coast. To ascertain whether mortality might be associated with morphological features of the shells, we estimated fluctuating asymmetry (FA) of hatchery/nursery-produced oysters kept in culture bags in the intertidal. FA is defined as random variations from a perfect symmetry plane (bilateral or radial) in some morphological trait. FA increases with disruption of homeostasy during ontogeny and has both genetic and environmental determinants. We found significant differences in FA between dead and live oysters in two of our three study groups. Therefore, lack of developmental stability may be involved in the summer mortality syndrome of oysters. We also found...
Tipo: Text Palavras-chave: Oyster; Mortality; Fluctuating asymmetry; Aquaculture.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-575.pdf
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